Research Article

Effect of trichostatin A on fertilization and embryo development during extended culture of mouse oocyte

Byung Chul Jee^a2a3, Jun Woo Jo^a2, Jung Ryeol Lee^a2, Chang Suk Suh^a1a3 c1, Seok Hyun Kim^a3 and Shin Yong Moon^a3

^a1 Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, 300 Gumi-dong Bundang-gu Seongnam, 463–707, Korea.

^a2 Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, Seongnam, 463–707, Korea

^a3 Department of Obstetrics and Gynecology, Seoul National University College of Medicine, Seoul, 110–744, Korea

Summary

We performed this study to investigate the effect of histone deacetylase inhibition during extended culture of in vitro matured mouse oocytes. In vitro matured mouse (BDF1) oocytes were cultured in vitro for 6, 12, and 24 h, respectively, and then inseminated. During in vitro culture for 6 and 12 h, two doses of trichostatin A (TSA), a histone deacetylase inhibitor, were added (100 nM and 500 nM) to the culture medium and the oocytes were then inseminated. During the 24-h in vitro culture, two doses of TSA were added (100 nM and 500 nM) to the medium and the oocytes were activated with 10 mM SrCl₂. After the 6-h culture, the fertilization rate was similar to that of the control group, but the blastocyst formation rate was significantly decreased. After the 12-h culture, both the fertilization and blastocyst formation rates were significantly decreased. After the 24-h culture, total fertilization failure occurred. In the oocytes cultured for 6 and 12 h, the fertilization and blastocyst formation rates did not differ between the TSA-supplemented and control groups. Although extended culture of the mouse oocytes significantly affected their fertilization and embryo development, TSA supplementation did not overcome their decreased developmental potential.

(Received May 24 2010)

(Accepted August 04 2010)

(Online publication January 27 2011)

Keywords:

• Extended culture;
• Histone deacetylase;
• In vitro maturation;
• Oocyte;
• Trichostatin A

Correspondence:

^c1 All correspondence to: Chang Suk Suh. Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital, 300 Gumi-dong Bundang-gu Seongnam, 463–707, Korea. Tel: +82 31 787 7251. Fax: +82 31 787 4054. e-mail: suhcs@snu.ac.kr