Zygote
- Zygote / Volume 19 / Issue 01 / February 2011, pp 67-70
- Copyright © Cambridge University Press 2010
- DOI: http://dx.doi.org/10.1017/S0967199410000146 (About DOI), Published online: 23 June 2010
Research Article
Ultraviolet radiation and handling medium osmolarity affect chimaerism success in zebrafish
M. Francisco-Simãoa2a3, J. Cardona-Costaa1 c1, M. Pérez-Campsa2 and F. García-Ximéneza2
a1 Laboratory of Animal Reproduction and Biotechnology (LARB-UPV), Universidad Politécnica de Valencia, Camino de Vera 14,46071 Valencia, Spain.
a2 Laboratory of Animal Reproduction and Biotechnology (LARB-UPV), Universidad Politécnica de Valencia, Camino de Vera 14,46071 Valencia, Spain.
a3 Faculty of Agriculture Sciences – Agostinho Neto University, Angola.
Summary
The effects of a predefined ultraviolet radiation dose (0.529 mW/cm2 for 30s) together with two different micromanipulation medium osmolarities (30 mOsm/kg vs 300 mOsm/kg) were tested on embryo survival at different developmental stages and on the somatic (skin) and germ-line chimaerism rates. Somatic (13%, 6/47 adults) and germ-line chimaerism (50% pigmented F1 larvae) were detected only in the UV-treated recipient embryos micromanipulated in a 300 mOsm/kg medium. From the results obtained, we concluded that the conditions cited above were the most suitable to improve somatic and germ-line chimaerism rates in zebrafish.
(Received October 09 2009)
(Accepted January 21 2010)
(Online publication June 23 2010)
Keywords:
Correspondence:
c1 All correspondence to: J. Cardona-Costa. Laboratory of Animal Reproduction and Biotechnology (LARB-UPV), Universidad Politécnica de Valencia, Camino de Vera 14,46071 Valencia, Spain. Tel: +34 963879433. Fax: +34 963877439. e-mail: cardona_costa_j@hotmail.com
