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  • Enzyme-linked immunosorbent assay with worm vomit and cercarial secretions of Schistosoma mansoni to detect infections in an endemic focus of Burkina Faso

Journal of Helminthology

Table of Contents - 2006 - Volume 80, Issue 01  

Cambridge Journals Online - CUP Full-Text Page

Research Article

Enzyme-linked immunosorbent assay with worm vomit and cercarial secretions of Schistosoma mansoni to detect infections in an endemic focus of Burkina Faso

M. Bahgata1 †, H. Sorghoa1a2, J.B. Ouédraogoa2, J.N. Podaa2, L. Sawadogoa3 and A. Ruppela1 c1



a1 Department of Tropical Hygiene and Public Health, University of Heidelberg, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany

a2 Institut de Recherche en Sciences de la Santé, Bobo-Dioulasso, Burkina Faso

a3 UFR-SVT, Université de Ouagadougou, Burkina Faso.

Article author query

Bahgat M [PubMed]  [Google Scholar]
Sorgho H [PubMed]  [Google Scholar]
Ouédraogo JB [PubMed]  [Google Scholar]
Poda JN [PubMed]  [Google Scholar]
Sawadogo L [PubMed]  [Google Scholar]
Ruppel A [PubMed]  [Google Scholar]

Abstract

Cercariae and adult Schistosoma mansoni were used to prepare, respectively, cercarial secretions (CS) and worm vomit (WoV). These were used as antigens in an enzyme-linked immunosorbent assay (ELISA) to test the IgG-reactivity of sera obtained in an S. mansoni-endemic area of Burkina Faso. Among the egg-excreting individuals (n=240), 94.6% reacted positively with WoV, but only 62.9% with CS, thus suggesting a high diagnostic sensitivity of WoV, but not of CS. Among those individuals without detectable eggs in two Kato-Katz thick smears from different stool specimens (n=215), the respective percentages of positive IgG reactivity were 78.1% and 63.3%. These positive reactions in the absence of detectable eggs are interpreted in terms of limited sensitivity of parasitological stool examinations. Optical density values in ELISA with CS, but not with WoV, correlated negatively with age, which may reflect decreasing exposure to cercariae in older individuals.

(Accepted June 17 2005)

Correspondence:

c1 Author for correspondence Fax: +49 6221 565948, E-mail: Andreas.Ruppel@urz.uni-heidelberg.de.

Footnotes

† Department of Medicinal Chemistry and Central Laboratory, Division of Pharmaceutical Industries and Drug Research, National Research Center, Dokki, Cairo, Egypt.