a1 London School of Hyglene and Tropical Medicine, Winches Farm Field Station, 395 Hatfield Road, St. Albans, Herts. ALA OXQ,
a2 Department of Morbid Anatomy, University College Hospital, University Street, London WC1E 6JJ, UK
Twenty-one mice were injected intramusclarly with 2000 Schistosoma mansoni schistosomula irradiated at 20 Krad and cryopreserved; three mice were killed on each of days 0, 2, 5, 9, 19, 28 and 44 days after infection and muscle from the site of injection in the left hind leg, the lungs and livers removed for histological examination. Schistosomula were seen in sections from the leg muscle from days 0 to 19 inclusive, in the lungs from day 2 to day 28 inclusive and in the livers from days 9 to 28 inclusive. Most schistosomula were seen in sections of the leg muscle with considerably fewer parasites occurring in the lungs and especially the livers. Granulomatous reactions comprising cosinophils, polymorphs, plasma cells and macrophages were first seen in the leg muscle on day 2, in the lungs on day 5 and in the liver on day 19. The peak infiammatory reactions appeared to occur between days 5 and 9, 9 and 19 and 28 and 44 respectively in the three tissues. The pathology is discussed in relation to the dose of irradiation required to attenuate the schistosomula for optimal immunogenicity.
(Accepted September 19 1984)
p1 Present address: Department of Ophthamology, Medical University of South Carolina, 171 Ashley Avenue, Charleston, SC 29425, USA