Epidemiology and Infection
- Epidemiology and Infection / Volume 99 / Issue 01 / August 1987, pp 143-147
- Copyright © Cambridge University Press 1987
- DOI: http://dx.doi.org/10.1017/S0950268800066954 (About DOI), Published online: 19 October 2009
Research Article
Comparison of procedures based upon Rappaport–Vassiliadis medium with those using Muller–Kauffmann medium containing Teepol for the isolation of Salmonella sp.
P. Vassiliadisa1, Ch. Mavromatia1, D. Trichopoulusa2, V. Kalapothakia2 and J. Papadakisa3
a1 The Hellenic Pasteur Institute, Athens 11521, Greece
a2 The Department of Hygiene and Epidemiology, University of Athens, Athens 11527, Greece
a3 Athens School of Hygiene, Athens 11521, Greece
Abstract
A total of 308 samples of different types were examined for the presence of salmonellas by means of three different procedures. The first consisted of preenrichment in buffered peptone water followed by enrichment in Rappaport-Vassiliadis medium (P/RV). The second differed only in that 1% Teepol was added to the pre-enrichment medium (PT/RV). In the third, buffered peptone water with 1% Teepol was followed by enrichment in Muller–Kauffmann tetrathionate broth also containing 1% Teepol (PT/MKT). The first of these combinations (P/RV) proved superior to the others both in terms of isolation rates and in the appearance of suspicious colonies.
(Accepted January 28 1987)
