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Zygote

Table of Contents - 2004 - Volume 12, Issue 04  


Chromosomal analysis of mouse spermatozoa following physical and chemical treatments that are effective in inactivating HIV

Kazuto Morozumi a1c1, Hiroyuki Tateno a2, Kaoru Yanagida a3, Haruo Katayose a1, Yujiroh Kamiguchi a2 and Akira Sato a1
a1 Department of Obstetrics and Gynecology, Fukushima Medical University, Fukushima, Japan.
a2 Department of Biological Sciences, Asahikawa Medical College, Asahikawa, Japan.
a3 Clinical Research Center, International University of Health and Welfare, Ootawara, Japan.

Article author query
morozumi k   [PubMed][Google Scholar] 
tateno h   [PubMed][Google Scholar] 
yanagida k   [PubMed][Google Scholar] 
katayose h   [PubMed][Google Scholar] 
kamiguchi y   [PubMed][Google Scholar] 
sato a   [PubMed][Google Scholar] 

Abstract

Human immunodeficiency virus (HIV) can be inactivated by heating at 56 °C for 30 min, treating with 50% ethanol at room temperature for 10 min, or treating with 2% sodium hypochlorite solution (NaClO) at room temperature for 60 min. Using a mouse model, we evaluated the risk of generating chromosome damage in spermatozoa following these treatments. The spermatozoa were all dead after the treatments. Although 41.3% of oocytes injected with ethanol-treated spermatozoa successfully activated, none of the oocytes injected with heated or NaClO-treated spermatozoa activated. When artificial stimulation with strontium was used, the fertilization of oocytes with heated or ethanol-treated spermatozoa was completely rescued. Sperm nuclei treated with NaClO neither decondensed nor developed to a male pronucleus. The incidences of structural chromosome aberrations in 1-cell zygotes derived from the heated spermatozoa (45.6%) and ethanol-treated spermatozoa (91.2%) were significantly higher than those in the matched controls (5.5% and 10.5%, respectively). Further study is needed to develop a methodology for the protection of spermatozoa against chromosome damage or the separation of damaged spermatozoa before intracytoplasmic sperm injection.

(Received September 23 2004)
(Accepted September 21 2004)


Key Words: Chromosome; Ethanol treatment; Heating; Mouse spermatozoa.

Correspondence:
c1 All correspondence to: K. Morozumi, Department of Obstetrics and Gynecology, Fukushima Medical University, 1, Hikarigaoka, Fukushima 960-1295, Japan. Tel: +81 24 5471290. Fax: +81 24 5483878. e-mail: morozumi@fmu.ac.jp

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