Epidemiology and Infection

Table of Contents - 2003 - Volume 131, Issue 01  


Survey of Vibrio cholerae O1 and its survival over the winter in marine water of Port of Osaka

K. MIYAGI a1a2c1p1, T. NAKANO a1, T. YAGI a2, M. HANAFUSA a2, S. IMURA a3, T. HONDA a4, Y. NAKANO a2 and K. SANO a1
a1 Department of Microbiology, Osaka Medical College, Takatsuki-shi, Osaka 569-8686, Japan
a2 Osaka Quarantine Station, Osaka-shi, Osaka 552-0021, Japan
a3 Kobe Quarantine Station, Kobe-shi, Hyogo 652-0866, Japan
a4 Research Institute for Microbial Diseases, Osaka University, Suita-shi, Osaka 565-0871, Japan

Article author query
miyagi k   [PubMed][Google Scholar] 
nakano t   [PubMed][Google Scholar] 
yagi t   [PubMed][Google Scholar] 
hanafusa m   [PubMed][Google Scholar] 
imura s   [PubMed][Google Scholar] 
honda t   [PubMed][Google Scholar] 
nakano y   [PubMed][Google Scholar] 
sano k   [PubMed][Google Scholar] 

Abstract

The survey of Vibrio cholerae O1 in marine area was carried out in the Port of Osaka, Japan in 1987–2001, and 51 V. cholerae O1 strains were isolated. All strains were identified to be of El Tor biotype, Ogawa serotype and classic Ubon Kappa-phage type, and were cholera toxin (CT)-negative and CT gene-negative. In order to clarify certain ecological aspects of V. cholerae O1 in the marine environment of the temperate zone, we performed molecular analysis of the isolated strains using pulsed-field gel electrophoresis (PFGE) with NotI and SfiI restriction enzymes. We found the indistinguishable strains by DNA analysis using PFGE with strains passed for 1 year, and also found the closely related strains with that passed for 3 and 12 years. Those results indicated that V. cholerae O1 can survive over one winter at least, and that it survives in marine water for a long time by undergoing continuous mutation.

(Accepted March 18 2003)


Correspondence:
c1 Department of Microbiology, Osaka Medical College, 2-7 Daigaku-machi, Takatsuki-shi, Osaka 569-8686, Japan.
p1 Present address: Kobe Quarantine Station, Kobe, Japan.

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