Zygote
- Zygote / Volume 4 / Issue 01 / February 1996, pp 7-9
- Copyright © Cambridge University Press 1996
- DOI: http://dx.doi.org/10.1017/S0967199400002835 (About DOI), Published online: 26 September 2008
Article
The effect of 6-dimethylaminopurine (6-DMAP) on DNA synthesis in activated mammalian oocytes
S. Leddaa11, P. Loia12, L. Boglioloa11, R.M. Moora13 and J. Fulka Jra1 c1134
a1 University of Sassari, Italy, Institute of Ziitechnics, Olmedo, Italy, The Babraham Institute, Cambridge, UK, and Institute of Animal Production, Prague, Czech Republic.
Summary
Mouse, sheep and bovine metaphase II oocytes were briefly preincubated in medium with 6-dimethyl-aminopurine (6-DMAP) and thereafter activated with ethanol before further culture in 6-DMAP supplemented medium. The presence of 6-DMAP enhances the efficiency of activation and the speed of pronuclear formation but has no effect on DNA synthesis. These results are discussed in the context of recently published data showing that the preincubation of condensed chromosomes with 6-DMAP blocks DNA synthesis when nuclei become reformed.
(Received November 01 1995)
(Accepted November 23 1995)
Key words
Correspondence:
c1 J. Fulka, Jr, Institute of Animal Production, CS-104 00 Prague 10 – Uhříněves, Czech Republic. Telephone: 42 267710659. Fax: 42 267710779.
Footnotes
1 University of Sassari, Department of Physiology, I-07100 Sassari, Italy
2 Institute of Zootechnics, I-07040 Olmedo, Italy
3 The Babraham Institute, Development and Differentiation Laboratory, Babraham, Cambridge CB2 4AT, UK.
4 Institute of Animal Production, CS-10400 Prague 10, Czech Republic
