a1 Department of Zoology, University of California, Davis, and Bodega Marine Laboratory, California, USA.
Summary Antibodies to sea urchin β-tubulin and mammalian heavy neurofilaments were to used the late events of fertilisation in the penaeoidean shrimp Sicyonia ingentis. The neurofilament antibody fortuitously stained centrosomes in eggs, as well as the subacrosomal region and acrosomal filament in sperm. The neurofilament antibody also stained a cortial site in eggs which was associated with the positioning of the mitotic spindle. During pronuclear migration, a large maternal microtubule during syngamy, while the sperm centrosomes formed the poles of the first mitotic spindle. Colcemid treatment modulated the size of the mitotic spindle and blocked pronuclear migration.
c1 P.L. Hertzler, Department of Zoology, Duke University, 234 Bio-Sci Box 90325, Durham, NC 27708-0325, USA. Telephone: (919) 684-2693. Fax: (919) 684-6168.