a1 Department of Parasitology, School of Medicine, Hiroshima University, Minami Ku, Hiroshima 734, Japan
The enzyme-linked immunosorbent assay (ELISA) and immunoelectrophoresis (IEP) were used for the serodiagnosis of larval Anisakis simplex infections in man and immunized rabbits. Sephacryl S-300 gel filtration was used for separating crude antigen. Four fractions were obtained. Sera from patients with other helminth infections sometimes cross reacted with Anisakis larval antigens. With IEP, crude antigen is more sensitive than purified antigens. With ELISA, the third fraction is the most sensitive for detecting antibodies to Anisakis larvae in the sera of humans and immunized rabbits.
(Accepted May 25 1994)
p1 Hospital la Paz, Servicio de Farmacia, Paseo de la Castellana 261, 28046 Madrid, Spain.