Epidemiology and Infection

Table of Contents - 2002 - Volume 129, Issue 01  


Partial VP1/2A gene sequence based molecular epidemiology of wild type 1 poliovirus isolates from some parts of India

R.  ANAND  a1, D.  GHOSH  a2, A. V.  BHUPATIRAJU  a2, S.  BROOR  a2, S. T.  PASHA  a1, S.  KHARE  a1, M.  KUMAR  a1, K. K.  DUTTA  a1 and A.  RAI  a1 c1
a1 Division of Biochemistry and Biotechnology and Microbiology, National Institute of Communicable Diseases, Delhi, India
a2 Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India

Abstract

Genomic variability within the sequences of VP1/2A junction among polioviruses from across the globe has revealed the existence of several endemic genotypes and their epidemiological inter-relationships; but such data on Indian isolates are scanty. The present work was intended to ascertain the persistence and transmission pattern of different genotypes of wild type 1 polioviruses circulating in India. Forty-eight wild type 1 poliovirus isolates obtained from different parts of India during 1996–8 were subjected to RT–PCR and nucleotide sequencing using M13 tailed primers. A 293 base pair region was amplified and sequenced for genetic variation study. Considering the 15% divergence of the sequences from Sabin 1, the isolates from six different states of India confirmed a single dominant genotype 4. Phylogenetic analysis revealed the circulation and active inter-state transmission of many genetically distinct strains of wild poliovirus type 1 belonging to genotype 4. This warrants the need for insisting on more efficient surveillance mechanisms so as to assess the impact of an extensive pulse polio immunization programme in India.

(Accepted January 22 2002)


Correspondence:
c1 Author for correspondence: Division of Biochemistry and Biotechnology, National Institute of Communicable Diseases, 22, Shamnath Marg, Delhi: 110054, India.

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